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Flotation cell necrosis

Barge flotation, 2 107 cells were resuspended for 30 min at 4 C in 0.5 ml of TENT [50 mM Tris (pH 7.4) 150 mM NaCl 5mM EDTA 0.5% Triton X-100], supplemented with phosphatase and protease inhibitors, and extracted on ice for 30 min. For Raft and Barge flotation, cell lysates were adjusted to 50% with Optiprep (Nycomed, Oslo)

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